| 书目名称 | Electron Probe Microanalysis |
| 副标题 | Applications in Biol |
| 编辑 | Karl Zierold,Herbert K. Hagler (Associate Professo |
| 视频video | |
| 丛书名称 | Springer Series in Biophysics |
| 图书封面 |  |
| 描述 | The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techn |
| 出版日期 | Conference proceedings 1989 |
| 关键词 | X-ray; cells; diagnosis; spectroscopy; tissue |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-642-74477-8 |
| isbn_softcover | 978-3-642-74479-2 |
| isbn_ebook | 978-3-642-74477-8Series ISSN 0932-2353 Series E-ISSN 1868-2561 |
| issn_series | 0932-2353 |
| copyright | Springer-Verlag Berlin Heidelberg 1989 |
发表于 2025-3-21 16:43:08
