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Titlebook: Electrochemical Sensors in Immunological Analysis; T. T. Ngo Book 1987 Springer Science+Business Media New York 1987 Antigen.enzymes.labor

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发表于 2025-3-21 16:45:50 | 显示全部楼层 |阅读模式
书目名称Electrochemical Sensors in Immunological Analysis
编辑T. T. Ngo
视频video
图书封面Titlebook: Electrochemical Sensors in Immunological Analysis;  T. T. Ngo Book 1987 Springer Science+Business Media New York 1987 Antigen.enzymes.labor
描述The development of radioimmunoassay (RIA) by R.S. Yalow and S.A. Berson in 1959 opens up a new avenue in ultra­ sensitive analysis of trace substances in complex biological systems. In recognition of the enormous contributions of RIA to basic research in biology and to routine clinical tests in laboratory medicine, R.S. Yalow, the co-developer of RIA, was awarded, in 1977, the Nobel Prize for Medicine and Physiology. The basic principle of RIA is elegantly simple. It is based on a specific, competitive binding reaction between the analyte and the radio-labeled analog of the analyte for the specific antibody raised to the analyte. The combination of high specificity and affinity of an antibody molecule makes it a very versatile analytical reagent capable of reacting specifically with analytes at a very low concentration in a complex solution such as serum. The sensitivity of RIA is provided by using a radioactive tracer.
出版日期Book 1987
关键词Antigen; enzymes; laboratory medicine; physiology; protein; reaction
版次1
doihttps://doi.org/10.1007/978-1-4899-1974-8
isbn_softcover978-1-4899-1976-2
isbn_ebook978-1-4899-1974-8
copyrightSpringer Science+Business Media New York 1987
The information of publication is updating

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Selective Antibody Responsive Membrane Electrodes,plines. Although there exist well-established assays, alternative methods are still being sought. A considerable advance has been made in Potentiometric immuno-sensors alone. The method described in this chapter is different from straight forward enzyme labeled immuno techniques (e. g., Meyerhoff an
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Immuno-Potentiometric Sensors with Antigen Coated Membrane,ive sensors (biosensors), which include enzyme sensors, microbial sensors, and immunosensors (Aizawa, 1983; Suzuki, 1984). An enzyme sensor is the union of an enzyme, that is a biological catalyst which acts sensitively and specifically to almost all organic and inorganic componds in nature, with an
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Homogeneous Voltammetric Immunoassay of Haptens, a powerful technique for the analysis of a wide range of biologically important compounds. When a foreign species referred to as the antigen is recognised by an organism it starts immediately to produce an antibody that has a large binding constant for the various chemically distinctive elements of
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Immunoassay by Differential Pulse Polarography and Anodic Stripping Voltammetry,ity of immunoassay in diagnostic medicine is well documented, and a number of routine clinical methods have been established for the determination of a wide variety of common antigens (Landon and Moffar, 1976). However, immunoassay has only recently gained prominence within the analytical community
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Separation-Free (Homogeneous) Amperometric Immunoassay Using an Apoenzyme as the Label, 1984; Oellerich, 1984; Ngo, 1985): (1) separation-free, also known as homogeneous system in which the physical separation of unbound ligand-indicator conjugates from that bound to the antibody is not required; (2) separation-required, also known as heterogeneous system, in which an antibody bound l
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Digoxin Homogeneous Enzyme Immunoassay with Amperometric Detection,highly sensitive and selective means for quantitating an analyte. The applications of EIAs are numerous and ever-expanding (Jarvis, 1979). The sensitivity of EIAs is as high as that of radioimmunoassays (Maggio, 1980) which have detection limits ranging from 1-500 fmol/mL assay fluid (Oellerich, 198
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