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Titlebook: Eicosanoid Protocols; Elias A. Lianos Book 1999 Humana Press 1999

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Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry,he first steps in the conversion from arachidonic acid. A cDNA for cyclooxygenase was first cloned from sheep seminal vesicles in 1988 (.,.). In 1991, we and others reported the cloning of a second isoform of cyclooxygenase, now known as COX-2, that is rapidly induced in response to growth factors a
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Assessment of Cyclooxygenase Protein Expression by Western Blotting, an unknown complex protein sample. Electrophoresis is used to separate complex mixtures of proteins. Denaturing discontinuous one-dimensional gel electrophoresis separates proteins only based on molecular size as they move through a SDS-polyacrylamide gel (SDS-PAGE) toward the anode with the smalle
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Enzyme immunoassays (EIA) of Eicosanoids,um), tissue, urine, or culture media. Both radioimmunoassay and enzyme immunoassay techniques for the measurement of eicosanoids are based on the principles of the competitive substrate binding assay. More specifically, these assays involve the competition (for binding sites on an antibody) of a kno
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Transcellular Regulation of Eicosanoid Biosynthesis,ts that are generated by individual cell types in vitro. In vivo scenarios encountered in inflammation, thrombosis, atherosclerosis, asthma, and other multicellular events encompass an array of cell–cell interactions as well as a complex interplay of factors that include adhesion molecules, cytokine
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