书目名称 | ELISA |
副标题 | Methods and Protocol |
编辑 | Robert Hnasko |
视频video | http://file.papertrans.cn/301/300410/300410.mp4 |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshoo |
出版日期 | Book 2015 |
关键词 | Enzyme-Linked Immunosorbent Assay; antibody; hybridoma technology; immunoassay strategies; monoclonal an |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-2742-5 |
isbn_softcover | 978-1-4939-5385-1 |
isbn_ebook | 978-1-4939-2742-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2015 |