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Titlebook: ELISA; Methods and Protocol Robert Hnasko Book 2015 Springer Science+Business Media New York 2015 Enzyme-Linked Immunosorbent Assay.antibod

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书目名称ELISA
副标题Methods and Protocol
编辑Robert Hnasko
视频videohttp://file.papertrans.cn/301/300410/300410.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: ELISA; Methods and Protocol Robert Hnasko Book 2015 Springer Science+Business Media New York 2015 Enzyme-Linked Immunosorbent Assay.antibod
描述.This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshoo
出版日期Book 2015
关键词Enzyme-Linked Immunosorbent Assay; antibody; hybridoma technology; immunoassay strategies; monoclonal an
版次1
doihttps://doi.org/10.1007/978-1-4939-2742-5
isbn_softcover978-1-4939-5385-1
isbn_ebook978-1-4939-2742-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

书目名称ELISA影响因子(影响力)




书目名称ELISA影响因子(影响力)学科排名




书目名称ELISA网络公开度




书目名称ELISA网络公开度学科排名




书目名称ELISA被引频次




书目名称ELISA被引频次学科排名




书目名称ELISA年度引用




书目名称ELISA年度引用学科排名




书目名称ELISA读者反馈




书目名称ELISA读者反馈学科排名




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Affinity Purification of Antibodies,n crude form for the detection of target antigens by immunoassay. However, it is advantageous to use purified antibody in defined quantity to facilitate assay reproducibility, economy, and reduced interference of nonspecific components as well as improved storage, stability, and bio-conjugation. Alt
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Indirect ELISA,d surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries. This technique utilizes an enzyme-linked antibody binding to a surface-attached antigen. Subsequently, a substra
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Direct ELISA,of an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools. Traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with
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,ELISpot and DC-ELISpot Assay to Measure Frequency of Antigen-Specific IFNγ-Secreting Cells,d with a specific anti-analyte antibody. In response to the stimulation, cells release cytokines that are bound to the anti-analyte antibody. After a washing step, which removes the cells from the wells, the location of the cytokine-releasing cell is visualized by an enzyme-labeled detection antibod
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