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Titlebook: E. coli Gene Expression Protocols; Peter E. Vaillancourt Book 2003 Springer Science+Business Media New York 2003 Promoter.Proteomics.biosy

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Cold-Inducible Promoters for Heterologous Protein Expression,: membrane fluidity decreases, which interferes with transport and secretion, the secondary structures of nucleic acids are stabilized, which affect the efficiencies of mRNA transcription/translation and DNA replication, and free ribosomal sub-units and 70S particles accumulate at the expense of pol
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Maltose-Binding Protein as a Solubility Enhancer,ggregates known as inclusion bodies. Although it is sometimes possible to convert aggregated material into native, biologically-active protein, this is a time consuming, labor-intensive, costly, and uncertain undertaking (.). Consequently, many tricks have been employed in an effort to circumvent th
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Discovery of New Fusion Protein Systems Designed to Enhance Solubility in ,,valent application is the use of fusion proteins to improve protein expression in . and provide convenient methods for affinity-based protein purification. In many instances, when a foreign protein is overexpressed at high levels in ., the majority of the protein is present as insoluble inclusion bo
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