Titlebook: E. coli Plasmid Vectors; Methods and Applicat Nicola Casali,Andrew Preston Book 20031st edition Springer Science+Business Media New York 20

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Cost Standards for Efficient Competition,h (.). In bacterial cells, λ-phage employs one of two pathways of replication: lytic or lysogenic. Commonly, λ-phage vectors replicate via the lytic pathway. During lytic growth, the viral DNA is replicated manifold, a large number of phage gene products are synthesized, and progeny phage particles

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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/300217.jpg

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3.524he terminal sequences as well as the size of the amplified fragment and, in this way, provides freedom from the problems associated with location and abundance of restriction enzyme recognition sites.

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Lorrie Miller,Biljana C. Fredriksenvector will both grow on appropriate selective medium; therefore, screening is almost always necessary to select colonies containing the recombinant plasmid and, in some cases, with the correct orientation of insert.