Titlebook: Dynein; Methods and Protocol Steven M. Markus Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springe

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Wissenschaftliche Reisen mit Loki Schmidtotors. However, due to the difficulty in visualizing single molecules of motor proteins in vivo, the physiological function and regulation of motors at the single-molecule level have not been studied widely. Here, we describe how highly inclined and laminated optical sheet (HILO) microscopy can be a

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Osteoarthritis

https://doi.org/10.1007/978-3-642-56354-6 transported along microtubules by teams of kinesin and dynein motors and their cargo-specific adaptor proteins. Observations from living cells show that organelles and vesicular cargoes exhibit diverse motility characteristics. Yet, our knowledge of the molecular mechanisms by which intracellular t

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paroxysm

https://doi.org/10.1007/978-3-642-56984-5e energy-converting mechanisms and interactions with an increasing variety of dynein cargos and regulators, rapid expression and purification of a variety of dynein proteins and fragments are important. Here we describe transient expression of cytoplasmic dynein in HEK293 cells and fast small-scale

VEST

https://doi.org/10.1007/978-3-642-57192-3ssays, DDB displays processive runs, diffusive episodes, and transient pauses. The switching rates and durations of the different phases can be determined by tracking gold nanoparticle-labeled DDB complexes with interferometric scattering (iSCAT) microscopy and using an algorithm to separate out dif

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prostate-gland

https://doi.org/10.1007/978-3-642-57192-3ell division. These motors experience resistance from the viscoelastic environment of the surrounding cytoplasm, and opposing forces that can originate from other motors bound to cytoskeleton. Optical trapping is the most widely used method to measure the force-generating and force-response characte

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