| 书目名称 | Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition |
| 编辑 | Yogambigai Velmurugu |
| 视频video | |
| 概述 | Nominated as an outstanding PhD thesis by the University of Illinois at Chicago.Describes a novel approach combining the laser T-jump technique with unique sets of fluorescent probes to unveil previou |
| 丛书名称 | Springer Theses |
| 图书封面 |  |
| 描述 | .Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins. .Many cellular processes involve special proteins that bind to specific DNA sites with high affinity. How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific recognition. However, the dynamics of DNA deformations have been difficult to capture, thus obscuring our understanding of recognition mechanisms. .The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually |
| 出版日期 | Book 2017 |
| 关键词 | Dynamics of protein DNA interactions; Laser temperature-jump spectroscopy; Protein-induced DNA bending |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-319-45129-9 |
| isbn_softcover | 978-3-319-83218-0 |
| isbn_ebook | 978-3-319-45129-9Series ISSN 2190-5053 Series E-ISSN 2190-5061 |
| issn_series | 2190-5053 |
| copyright | Springer International Publishing AG, part of Springer Nature 2017 |
发表于 2025-3-21 16:37:21
