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Titlebook: Drosophila Oogenesis; Methods and Protocol Diana P. Bratu,Gerard P. McNeil Book 2015 Springer Science+Business Media New York 2015 RNA-prot

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https://doi.org/10.1007/978-3-642-98102-9e switches, which furthers our knowledge about cell cycle control machinery and sheds new light on potential cancer treatments. Here, we give a brief summary of cell cycle switches, the associated signaling pathways and factors, and the detailed experimental procedures used to study the cell cycle switches.
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Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Move. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.
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https://doi.org/10.1007/978-3-642-97360-4nalyzed through microscopy or biochemical and molecular biology techniques. Here we describe the isolation of ovarian tissues, techniques to enrich for egg chambers at distinct developmental stages, preparation of protein and nucleic acid extracts, and preparation for microscopic analysis of fixed tissues.
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Einmalgabetherapie mit Valproinsäurees an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in . egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.
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Vom Vertriebsingenieur zum Market-Ing.pproach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond . ovaries.
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