Titlebook: Doubled Haploid Technology; Volume 1: General To Jose M. Segui-Simarro Book 2021 Springer Science+Business Media, LLC, part of Springer Nat

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Methods for Chromosome Doublingion and chromosome doubling of induced haploids are the two main steps of doubled haploid creation. Both steps have their own complexities. Chromosome doubling of induced haploids may happen spontaneously, although usually at a low rate. Therefore, artificial/induced chromosome doubling of haploid c

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Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheatd to conventional line production, which requires several cycles of self-pollination. However, in durum wheat (. subsp. . Desf.), the low efficiency of green plant regeneration and the very high frequency of albino plants hinder the application of this technique..We observed the success of using gyn

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In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheatthe development of genetically homozygous lines in one generation, whilst in conventional breeding programmes, the development of homozygous lines requires more generations. The present chapter describes an efficient protocol for DH plant production in spelt wheat genotypes using in vitro anther cul

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Production of Wheat Doubled Haploids Through Intergeneric Hybridization with Maize high efficiencies. Wheat and maize donor plants are raised in environmentally controlled greenhouses until crossing. Before anthesis, wheat spikes are emasculated and then pollinated with maize. Auxin is applied to each individual wheat floret 1 day after pollination. About 2 weeks after crossing,