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Titlebook: Doubled Haploid Technology; Volume 3: Emerging T Jose M. Segui-Simarro Book 2021 The Editor(s) (if applicable) and The Author(s), under exc

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Applications of Impedance Flow Cytometry in Doubled Haploid Technologynumerous factors affecting its success and efficiency. Here we present concepts and examples about how critical success factors can be identified throughout a DH protocol and an early microspore response monitored by simple impedance flow cytometry (IFC) measurements, which will help to optimize each step of an androgenesis-based DH protocol.
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Doubled Haploid Parthenogenetic Production of Melon ‘Piel de Sapo’or chromosome doubling of haploid plants of ‘Piel de Sapo’ genotypes. ‘Piel de sapo’ genotype has lower efficiencies during the process in comparison with other genotypes, for instance, of the . subspecies. Nevertheless, the doubled haploid lines produced have a great potential as pure parentals for hybrid seed production.
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William J. V. Neill,Hanns-Uve Schwedlerd haploid induction including palm selection, anther isolation, pretreatment, and culture initiation, up to plant regeneration and thereafter acclimatization of the regenerated plants, is described. Furthermore, the protocol for testing the anther-derived plants for the ploidy level is also presented.
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Book 2021 to produce DHs in different species through different in vivo and in vitro approaches. Volume 3 looks at the emerging tools used in DH technology; protocols based on anther culture, in vitro gynogenesis and hybridization with irradiated pollen for DH production in cucubits such as cucumber, melon,
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Political Economy of Developmentliferate forming calli. The calli produced are isolated from anthers and transferred first to a liquid medium and then to a solid medium to induce organogenesis. Organogenic shoots will eventually give rise to entire DH plants.
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Using History, Making British Policyg program is still limited. As a possible alternative technique, gynogenesis (unfertilized ovule/ovarium cultures) switches on to produce haploid plants in some . species. In the . genus, gynogenesis has been one of the most studied and popular DH techniques and presented remarkable results in recent years.
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