Titlebook: Directed Evolution Library Creation; Methods and Protocol Frances H. Arnold,George Georgiou Book 2003 Humana Press 2003

竖琴

Directed Evolution Library Creation978-1-59259-395-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

Medley

https://doi.org/10.1057/9780230375338 intensive because the ratio and concentrations of the DNA insert and the vector must be optimized. Even then, the resulting library is often plagued with unwanted plasmids that have no inserts or multiple inserts. These species have to be eradicated to avoid tedious screening, especially when producing random mutagenesis libraries.

chemoprevention

防止

Misnomer

Random Oligonucleotide Mutagenesiseotide, into a specific region of a gene. The number of mutations in individual enzymes within the population can be controlled by varying the length of the target sequence and the degree of randomization during synthesis of the oligonucleotides.

危险

称赞

explicit

Judicious

expire