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Titlebook: Directed Enzyme Evolution; Screening and Select Frances H. Arnold,George Georgiou Book 2003 Humana Press 2003

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High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidasesroad variety of natural sources, most peroxidases use heme or vanadium as a cofactor at the redox active site, while some bacterial peroxidases function without a metal cofactor (.,.). Peroxidases catalyze a wide variety of oxidative reactions, some of which are used in industrial and biotechnologic
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High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidaseson without a metal cofactor (.,.). Peroxidases catalyze a wide variety of oxidative reactions, some of which are used in industrial and biotechnological applications. An activity assay that can be used in a high-throughput fashion becomes important for the engineering of new and improved peroxidases.
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Müfit Bahadir,Harun Parlar,Michael Spiteller acids to stability are still in their infancy. Therefore, evolutionary methods for increasing protein stability are of great interest. In such approaches, large protein libraries are searched for stabilized variants by a screening or, ideally, a selection technique.
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