Titlebook: Differential Display Methods and Protocols; Peng Liang,Jonathan D. Meade,Arthur B. Pardee Book 2006Latest edition Humana Press 2006 DNA.Mi

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ROBOT2013: First Iberian Robotics Conferenceke dot blots, many different experiments can be spotted on a single vertical array, allowing single genes to be compared across many conditions. Vertical arrays have two additional advantages over dot blots. First, they are printed on glass slides, allowing the use of low-volume, high-concentration

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https://doi.org/10.1007/978-3-031-21062-4ibe a flexible method for computer-assisted ranking of expression patterns in data from DD experiments. The method is based on a pairwise alignment and comparison of the quantitative trace data with respect to specific expression patterns defined by the investigator. The observed patterns are ranked

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João Macedo,Lino Marques,Ernesto Costaquence databases with temporal and spatial expression profiles. To meet this need, we have developed a powerful computer program (GenEST), which links cDNA sequence data (including EST sequences) with transcript profiles revealed by cDNA-amplified fragment length polymorphism (AFLP). cDNA-AFLP is a

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L. Beji,A. Abichou,P. Joli,M. Pascal reaction (DDRT-PCR) procedure to compare gene expression in spleens of infected and uninfected mice. In this study, we identified a novel erythroid-specific gene that was differentially expressed as a result of prion infection. We were able to demonstrate that a decrease in the expression levels of

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F. L. Chernousko,N. N. Bolotnikmethod on biomedical research, there has been a lack of automation of DD technology to increase its throughput and accuracy for a systematic gene expression analysis. Most of previous DD work has taken a “shotgun” approach of identifying one gene at a time, with limited polymerase chain reaction (PC

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https://doi.org/10.1007/978-3-7091-2666-0G2) and to control apoptosis in response to abnormal proliferative signals and stress, including DNA damage, is considered to be important for its tumor-suppression function. p53 is a transcription factor that binds to DNA in a sequence-specific manner to activate transcription of target genes. In t

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Erika Ottaviano,Gianni Castellimembrane of cells in ovarian follicles. This article outlines differential display (DD) protocols and associated methods that have been used to discover more than 30 genes that are expressed in the rat ovary during the ovulatory process. Details are provided regarding the methods for total RNA extra

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Makoto Kaneko,Mitsuru Higashimori. In an effort to better understand the molecular nature of this retinal clock, a differential display (DD) screen was performed to isolate a gene with high amplitude circadian rhythmicity in the Xenopus retina. A novel gene expressed in the early evening in photoreceptor cells was isolated and name