| 书目名称 | Difference Gel Electrophoresis (DIGE) |
| 副标题 | Methods and Protocol |
| 编辑 | Rainer Cramer,Reiner Westermeier |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past and future advances of protein purification and analysis methods. DIGE, being able to quantify proteins in their intact form, is one of a few methods that can facilitate this type of analysis and still provide the protein isoforms in an MS-compatible state for further identification and characterization with high analytical sensitivity. .Differential Gel Electrophoresis: Methods and Protocols. introduces the concept of DIGE and its advantages in quantitative protein analysis. It provides detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics. Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduc |
| 出版日期 | Book 2012 |
| 关键词 | 2D DIGE; biomarker discovery; mass spectrometry; proteomic profiling; quantitative proteomics |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-61779-573-2 |
| isbn_softcover | 978-1-4939-6205-1 |
| isbn_ebook | 978-1-61779-573-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC 2012 |
发表于 2025-3-21 19:38:55
