Titlebook: Difference Gel Electrophoresis; Methods and Protocol Kay Ohlendieck Book 2018 Springer Science+Business Media LLC 2018 DIGE.fluorescence im

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https://doi.org/10.1007/978-94-009-8808-8ructural and functional heterogeneity to the proteome. One needs to consider this aspect while studying changes in abundance or activities of proteins in response to any physiological stimulus. Abundance changes in components of a given proteome can be best visualized and quantified using electropho

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Quadrilingual Economics Dictionaryt for protein spots visualized on 2D polyacrylamide gels. This is particularly important for samples, which need to be compared without the availability of replicates and thus cannot be studied using differential gel electrophoresis (DIGE). CoFGE corrects for gel-to-gel variability by co-running wit

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Quadrilingual Economics Dictionary standard DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitive and accur

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B. Reulet,D. E. Prober,W. Belzigsment. It is based on two-dimensional gel electrophoresis (2-DE) separation of fluorescently labeled protein extracts. The tagging procedures are designed to not interfere with the chemical properties of proteins with respect to their p. and electrophoretic mobility, once a proper labeling protocol