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Titlebook: Difference Gel Electrophoresis; Methods and Protocol Kay Ohlendieck Book 2023Latest edition The Editor(s) (if applicable) and The Author(s)

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Comparative 3-Sample 2D-DIGE Analysis of Skeletal Muscles muscles are characterized by a distinctive combination of contractile cells with differing physiological and biochemical properties, it is essential to determine specific differences in the protein composition of fast, slow, and hybrid fibers. Fluorescence two-dimensional difference gel electrophor
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Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkerse comparative and mass spectrometry-based proteomic analysis of body fluids can be particularly instrumental for the targeted identification of novel protein biomarkers with pathological relevance. In this respect, new research efforts in biomarker discovery focus on the systematic mapping of the hu
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DIGE Analysis of Animal Tissuesn in complex mixtures. The technique addresses some of the drawbacks of conventional 2D polyacrylamide gel electrophoresis (2D-PAGE), offering improved sensitivity, more limited experimental variation, and accurate within-gel matching. 2D-DIGE is based on direct labeling of proteins with isobaric fl
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DIGE-Based Biomarker Discovery in Blood Cancersy to couple 2D-DIGE with additional downstream mass spectrometry-based techniques yields comprehensive workflows capable of identifying proteins of biological and clinical significance. The application of 2D-DIGE in blood cancer research has significantly contributed to the increasingly important in
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DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) biopsies are precious samples that can generally be acquired in very small amounts due to the invasive nature of the sample collection, mainly during surgery or biopsy. Subsequently, the amount of extracted protein can be, in many cases, very limited. The saturation 2D-DIGE technology has emerged a
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DIGE Analysis of Animal Tissuesdividual protein spot, intensities recorded at the different wavelengths are integrated and the ratio between volumes normalized to that of the internal standard. This provides an immediate appreciation of protein amount variations under the different conditions tested. In addition, proteins of inte
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Book 2023Latest edition and avoiding known pitfalls... ..Authoritative and cutting-edge, .Difference Gel Electrophoresis: Methods and Protocols, Second Edition. aims to ensure successful results in the further study of this vital field... .
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