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Titlebook: Diagnostic Bacteriology Protocols; Jenny Howard,David M. Whitcombe Book 19951st edition Humana Press 1995

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书目名称Diagnostic Bacteriology Protocols
编辑Jenny Howard,David M. Whitcombe
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: Diagnostic Bacteriology Protocols;  Jenny Howard,David M. Whitcombe Book 19951st edition Humana Press 1995
描述Enhanced public awareness of microbiological issues and increased governmental regulation have ensured that microbiology and bacteriology remain important, from the academic laboratory to a broad range of indus­ trial environments. Since the techniques used in bacteriology vary widely and have advanced rapidly of late, it has become increasingly desirable to gather a collection of up-to-date protocols from a comprehensive range of disciplines into a single volume. Diagnostic Bacteriology Protocols thus aims to provide a wide variety of up-to-date protocols for the identification and differentiation of bacteria from pure or mixed cultures, and for the study of bacteria at the genus through to subspecies levels. In addition, we aim to provide methods covering a range of complexities, some requiring little or no special equipment, others exploiting recent advances in sophisticated instrumentation or novel nucleic acid-based technology. We also present techniques to study a variety of aspects of bacteria—from the features of whole cells and their growth, through their enzyme activities and structural components, to nucleic ac- based analysis.
出版日期Book 19951st edition
版次1
doihttps://doi.org/10.1385/0896032973
isbn_ebook978-1-59259-533-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1995
The information of publication is updating

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Conrado C. Gonçalves,Carlos H. I. RamosThe technique of pulsed field gel electrophoresis (PFGE) enables researchers to separate linear DNA fragments of up to 10 Mb. The technique was first described by Schwartz and Cantor (.) and differs from conventional gel electrophoresis by requiring the DNA molecules periodically to change their direction of migration.
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Conrado C. Gonçalves,Carlos H. I. RamosTotal DNA restriction pattern analysis is one of the techniques commonly used to identify and group bacteria (.–.). This technique can be used to separate species belonging to the same genus (.,.) or to distinguish highly similar bacterial strains within the same species or subspecies (.–.).
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The Direct Immunofluorescent Filter Technique (DIFT),Many areas of analytical microbiology that deal with biological materials (e.g., medicine and the food, water, and pharmaceutical industries) need methods of detecting and identifying microorganisms that can give results in a few hours rather than the many days required for conventional methods.
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Antibiotic Resistance Profiling,a bacterium is tested for resistance, it should be compared, quantitatively with a standard. This standard may be the resistance of a wild-type strain, a typical strain, or a “type” strain, appropriate to the test strain.
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Pyrolysis Mass Spectrometry,tra represent transient bacterial “fingerprints” that can be compared mathematically for relatedness. The method has potential for identification, classification, and typing of bacteria with a high degree of resolution and speed.
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