Titlebook: Diagnosis of human peroxisomal disorders; A handbook Frank Roels (Professor of Human Anatomy and Embryo Book 1995 Springer Science+Business

rectum

Immunocytochemical localization of peroxisomal proteins in human liver and kidney,he three .-oxidation enzymes, alanine: glyoxylate aminotransferase and a peroxisomal membrane protein) in human liver biopsies are described. We present a protocol for immunolabelling on ultrathin and semithin sections from the same tissue block, with protein A-colloidal gold as a reporter system. F

错

Liver and chorion cytochemistry,nlarged liver macrophages containing lipid droplets insoluble in acetone and nhexane as well as polarizing inclusions formed by stacks of trilamellar sheets are of diagnostic value in peroxisomal disorders. Methods are presented for evaluating these structures by light microscopy; trilamellar inclus

Explosive

gerrymander

Secondary alterations of human hepatocellular peroxisomes,acquired or congenital non-peroxisomal diseases are reviewed. Secondary peroxisomal changes are observed in steatosis, hepatitis and cirrhosis induced by various agents (viruses, alcohol, drugs, etc.), in cholestasis, in hepatomas, in extra-hepatic cancer with or without liver metastasis, in extrahe

forager

树上结蜜糖

Book 1995different peroxisomal disorders has beendescribed. The best known conditions include Zellweger syndrome,rhizomelic chondrodysplasia punctata and X-linked adrenoleukodystrophyand, in the most recent edition of .The Metabolic and MolecularBasis Inherited. .Disease., edited by Scriver and colleagues,mo

严厉批评

Measurement of very long-chain fatty acids, phytanic and pristanic acid in plasma and cultured fibrasma and fibroblasts. A survey is given of the concentrations of very long-chain fatty acids, pristanic and phytanic acid in plasma and fibroblasts from control subjects and all patients investigated so far in our laboratory.

船员

Measurement of dihydroxyacetone-phosphate acyltransferase (DHAPAT) in chorionic villous samples, blnctata and DHAPAT-deficiency. We describe a straightforward and accurate assay allowing the activity of DHAPAT to be measured reliably in chorionic villus samples, blood cells, cultured skin fibroblasts, cultured chorionic villus fibroblasts and cultured amniocytes.

保守党

Liver and chorion cytochemistry,ions are only detected by electron microscopy. Macrophage features are preserved in archival paraffin blocks. In adrenal cortex, insoluble lipid, polarizing inclusions and trilamellar structures should be looked for. The stains are easily reproducible, and all reagents are commercially available.

Lipoprotein