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Titlebook: Diagnosis of Human Viruses by Polymerase Chain Reaction Technology; Yechiel Becker,Gholamreza Darai Book 1992 Springer-Verlag Berlin Heide

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书目名称Diagnosis of Human Viruses by Polymerase Chain Reaction Technology
编辑Yechiel Becker,Gholamreza Darai
视频video
丛书名称Frontiers of Virology
图书封面Titlebook: Diagnosis of Human Viruses by Polymerase Chain Reaction Technology;  Yechiel Becker,Gholamreza Darai Book 1992 Springer-Verlag Berlin Heide
描述The basis for the effective treatment and cure of a patient is the rapid diagnosis of the disease and its causative agent, which is based on the analysis of the clinical symptoms coupled with laboratory tests. Although rapid advance­ ments have been made in the laboratory diagnosis of virus diseases, the neces­ sary isolation of the causative virus from the clinical specimens is a relatively long procedure. Viruses which integrate into the cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses which exist in the clinical material in low concentrations are even more formidable to identify. Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (1985) led to a revolution in virus diagnosis. The peR technique was rapidly applied to the diagnosis of viruses in clinical material. Volume 1 of Frontiers of Virology provides new information on the advan­ tages of the use of the peR for the diagnosis of many human disease-causing viruses, as well as on some problems with its use.
出版日期Book 1992
关键词AIDS; CMV; Human Viruses; Humanpathogene Viren; PCR; RNA; Viren; Viruses; diseases; hepatitis; retroviruses; vi
版次1
doihttps://doi.org/10.1007/978-3-642-84766-0
isbn_softcover978-3-642-84768-4
isbn_ebook978-3-642-84766-0Series ISSN 1431-357X
issn_series 1431-357X
copyrightSpringer-Verlag Berlin Heidelberg 1992
The information of publication is updating

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Detection of Human Spumaviruses by Polymerase Chain Reactionhniques. The only serious problem encountered is the oversensitivity of this method that can lead to false-positive results due to laboratory contaminations. Controls and the complete physical separation of all reagents and equipment are required to avoid any contaminations.
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Ilaria Tarricone,Anita Riecher-Rösslerhniques. The only serious problem encountered is the oversensitivity of this method that can lead to false-positive results due to laboratory contaminations. Controls and the complete physical separation of all reagents and equipment are required to avoid any contaminations.
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Human Immunodeficiency Virus Type 1 (HIV-1) Detection by Polymerase Chain Reaction in Children of Ine second and third testing. I conclude that, in most cases, PCR will not detect the presence of HIV-1 proviral DNA at birth, but it is a powerful technique that can be used for the diagnosis of perinatal HIV-1 infection from the second month of life.
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Alexandra Boccarossa,Sébastien Fleurettivation of HCV. PCR based diagnostics, the rapid accumulation of HCV sequence information and the availability of cDNA/PCR generated cDNA clones for the expression of putative viral antigens has contributed greatly to our understanding of the structure and biology of HCV.
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Case Study: Cynthia’s Story: The Netherlandsg the earliest stages of central nervous system infection. The assay has also been used for the detection of HSV DNA in CSF samples from patients with HSE. The results are discussed and compared with those published elsewhere.
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Paul Bowie,Simon Paterson-Brownngement known to disrupt viral latency. Technical details of amplification, oligonucleotide hybridization, and semiquantitative EBV DNA analysis are described. A sensitive detection system of the Bam W/Bam Z rearrangement is also presented.
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