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Titlebook: Developmental, Physiological, and Functional Neurobiology of the Inner Ear; Andrew K. Groves Book 2022 The Editor(s) (if applicable) and T

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Xiao-gang Zhang,Cai-fu Yang,Cheng-jia Shange-specific marker genes for the design of transgenic models, and illuminated the inner ear molecular heterogeneity, both within and across cell types. Here we compare different methods of tissue- and cell-specific gene expression analyses, followed by a methodological overview of the use of the RiboTag mouse model for studying the inner ear.
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Book 2022d comprehensive, .Developmental, Physiological, and Function Neurobiology of the Inner Ear. is a valuable resource for scientists and researchers interested in learning more about this developing field. .
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Mice Enable Selective and Timed Ablation of Hair Cells in Postnatal Miceptor (also known as HB-EGF) is expressed under regulation of the . promoter. Because . is expressed by all hair cells (and relatively few other cells in the body), this mouse model permits the selective elimination of hair cells via 1–2 systemic injections of diphtheria toxin. This mouse line has be
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Molecular Tools to Study Regeneration of the Avian Cochlea and Utricler type I versus type II hair cells, and proximal versus distal location along the cochlea. We provide a method to quantitate cells within the sensory epithelium in 3D, leveraging vibratome sectioning and imaging methods that are presented in a companion chapter. Finally, we present the technique of
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In Vivo Analysis of Hair Cell Sensory Organs in Zebrafish: From Morphology to Functionan sensorineural hearing loss. This methods chapter serves as a guide to creating and studying novel zebrafish mutants, covering a range of topics from zebrafish breeding and propagation to embryo microinjections for targeted mutagenesis (morpholino, CRISPR-Cas9, and tol2-based transgenesis). Once t
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