Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2021

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In Situ Hybridization Techniques in the Homoscleromorph Sponge , the origin and early evolution of key animal features. Transcriptomic or genomic data acquired during the last 10 years have highlighted the conservation of most of the main genes and pathways involved in the development of the other metazoans. The next step is to determine how similar genetic tool

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Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Doublonstrate the power of fluorescence in situ RNA hybridizations to localize sites of specific RNA accumulation in whole mount embryo applications. We add to this technology the use of various probe-labeling technologies to colabel multiple RNAs in one application and we describe protocols for incorpor

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Gene Editing in the Ascidian , and Tail Nerve Cord Formation,in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian ..

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RNA Interference on Regenerating Holothurian Gut Tissues,rating tissues can elucidate potential effectors during this process. Here we describe an effective transfection protocol that allows the introduction of Dicer-substrate interference RNAs (DsiRNAs) for the modulation of gene expression and its characterization during regeneration.

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,Functional Studies of , Voltage-Gated Calcium Channel Activity,s primarily focused on their morphology and the development of genomic resources, . has received increased attention as a model for studying the evolution of nervous and sensory systems. This work is motivated by the fact that . features distinct behaviours and responses to environmental stimuli. Th

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A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna, as adaptations to the highly specialized aquatic interstitial habitat. While revealing much about the extreme phyletic diversity of meiofauna, the genome content of meiofaunal species could also characterize the phenotype of ancestral states as well as explain the origin and evolution of miniaturiz

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