Titlebook: Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis; Bin Zhuang Book 2018 Springer Nature Singa

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Book 2018armacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successfuldevelopment and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice..

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Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

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Introduction,d. Different kinds of approaches have their own advantages and application occasions. Microfluidic is a newly developed technology that aims at precisely manipulating fluidic of micro-scale. The microfluidic made it possible to integrate tradition genetic analysis process onto one single microchip f

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Construction of a General Platform for Capillary Electrophoresis,ainly referring to the glass chip for electrophoresis, with on-chip t sieving matrix and several reagents like buffer, coating solutions; and off-chip part, the instrument including optical system for detection, electronic system for controlling of all actuators and mechanical system for optical adj

ATRIA

Integrated Module for Automated DNA Extraction and Amplification,te the chip, was designed and fabricated. On-chip extraction was performed by twining long-chain nucleic acid molecules onto the filter paper. And the thermal cycles were then applied directly to the filter paper with DNA twined on for PCR amplification. To precisely manipulate on-chip fluidic and f

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Fully Integrated Genetic Analysis System,ated genetic analysis. The chips and the instrument were also upgraded correspondingly. The upgraded integrated chip is a compound material one that uses plastic chip for sample preparation and glass chip for capillary electrophoresis detection. The cheap plastic chip is disposable while the glass c

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Conclusions and Prospects for Future Work,apter and offer some prospects for future works. We hope the system would be able to integrate more modules, deal with more various kinds of samples and be applied in more areas. We would be quite happy if our research works do help more and more people.

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Book 2018ction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directl