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Titlebook: Deubiquitinases; Methods and Protocol Julie Maupin-Furlow,Mariola J. Edelmann Book 2023 The Editor(s) (if applicable) and The Author(s), un

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书目名称Deubiquitinases
副标题Methods and Protocol
编辑Julie Maupin-Furlow,Mariola J. Edelmann
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Deubiquitinases; Methods and Protocol Julie Maupin-Furlow,Mariola J. Edelmann Book 2023 The Editor(s) (if applicable) and The Author(s), un
描述.This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...Authoritative and cutting-edge, .Deubiquitinases: Methods and Protocols .aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
出版日期Book 2023
关键词DUB inhibitors; Next-generation phage display; poly-Ub chain; therapeutic opportunities v; innate immuni
版次1
doihttps://doi.org/10.1007/978-1-0716-2803-4
isbn_softcover978-1-0716-2805-8
isbn_ebook978-1-0716-2803-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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High-Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity,talling substrate translocation. With the proteasome as an approved anticancer target, Rpn11 is therefore an attractive point of attack for the development of new inhibitors, which requires robust biochemical assays to measure DUB activity. Here we describe a method for the purification of the Rpn8/
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Bioinformatical Approaches to the Discovery and Classification of Novel Deubiquitinases,an be used to identify high-confidence DUB candidates from pathogen genomes – even in the complete absence of biochemical or functional annotation. This chapter addresses bioinformatical DUB discovery approaches based on a previously published analysis of DUB evolution. The core set of bioinformatic
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Francesco Dramis,Giandomenico Fubellitalling substrate translocation. With the proteasome as an approved anticancer target, Rpn11 is therefore an attractive point of attack for the development of new inhibitors, which requires robust biochemical assays to measure DUB activity. Here we describe a method for the purification of the Rpn8/
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