Titlebook: Detection and Typing Strategies for Pathogenic Escherichia coli; Lucia Rivas,Glen E. Mellor,Narelle Fegan Book 2015 © The Authors 2015 201

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Book 2015tain strains among these groups are major public health concerns due to the severity of disease that they can cause. Methods to detect and isolate these pathogens from a variety of sources are constantly evolving. In addition, the accumulation of knowledge on these pathogens allows for improved intervention strategies.

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Isolation and Detection of Pathogenic , in Foods,r . pathotypes. However, popular methods such as Polymerase Chain Reaction (PCR) have limitations, including the inability to differentiate live cells from dead and the inhibition of PCR by complex media. One major limitation of all detection methods is the lack of sensitivity to directly detect the