Titlebook: Detection and Enumeration of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater; Marciane Magnani Book 2021 The Editor(s) (if

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P. H. Clodi,K. Ewe,K. Krentz,P. H. ClodiR) assay for simultaneous detection of . spp. . spp., . ., and . . on leafy greens. The nested mPCR assay provides a rapid, inexpensive, and simple approach for simultaneous detection of protozoan pathogens on fresh produce.

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P. H. Clodi,K. Ewe,K. Krentz,P. H. Clodi for the direct detection of the viability of . in food matrices, from evaluations of survival (in vitro), as well as of infectivity, pathogenicity, and virulence (in vivo). The methodology has already shown important results in previous studies, and new perspectives can be pointed out when consider

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https://doi.org/10.1007/978-3-642-79811-5ing viability for a long time in the environment and water. The diagnosis of this protozoan in water samples is difficult, mainly because of variability in the water physical parameters which makes the development of a standard technique difficult. Some advances in methodologies have been described

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,Übersichtstabellen I–V zur Diagnose,ted oocysts, and tachyzoites, a form of rapid multiplication found during the acute phase in blood and milk. Considering the importance of milk as a food, and its participation as a transmission patterns in outbreaks of toxoplasmosis, it is necessary to standardize a technique for recovery of . in t

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