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Titlebook: Dengue; Methods and Protocol Radhakrishnan Padmanabhan,Subhash G. Vasudevan Book 2014 Springer Science+Business Media, LLC 2014 RNA protein

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Luís Rosa,Fábio Silva,Cesar Analide the serotype of the antigen can be kept constant or alternated during the selection process depending on whether serotype-specific or cross-reactive antibodies are required. After the selection process, clones are screened, and specific clones are identified by phage ELISA and Western blot.
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Development and Application of Dengue Virus Reverse Genetic Systemsation and are useful for the screening of antiviral compounds. This article describes the development of dengue virus reverse genetic systems and protocols to manipulate the viral genome, recover infectious virus, and produce replicon-containing cell lines.
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Next-Generation Whole Genome Sequencing of Dengue Virusover the course of a single infection. The aim of this chapter is twofold: to describe the methodologies required for sample preparation prior to sequencing and to describe the bioinformatics analyses required for the resulting data.
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1064-3745 ation advice from the experts.Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year.
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Radhakrishnan Padmanabhan,Subhash G. VasudevanIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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