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Titlebook: Dendritic Cell Protocols; Elodie Segura,Nobuyuki Onai Book 2016Latest edition Springer Science+Business Media New York 2016 culture human

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书目名称Dendritic Cell Protocols
编辑Elodie Segura,Nobuyuki Onai
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Dendritic Cell Protocols;  Elodie Segura,Nobuyuki Onai Book 2016Latest edition Springer Science+Business Media New York 2016 culture human
描述.The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC). An introductoryreview provides an overview of recent advances in the characterization of DCsubsets in mouse and human. While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell progenitors from mouse, andthe purification of dendritic cells from human blood. Written in the highlysuccessful .Methods in Molecular Biology .series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls... ..Authoritative andcutting-edge,.  Dendritic CellProtocols, Third Edition .aims to ensure successful results in the furtherstudy of this vital field..
出版日期Book 2016Latest edition
关键词culture human dendritic cells; culture mouse dendritic cells; dendritic cell progenitors; human blood; D
版次3
doihttps://doi.org/10.1007/978-1-4939-3606-9
isbn_softcover978-1-4939-8092-5
isbn_ebook978-1-4939-3606-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Christoph Fuchs,Franziska J. Golenhofenke tyrosine kinase 3 ligand (Flt3L), either directly through a series of subcutaneous injections or by seeding with an Flt3L secreting murine melanoma. Flt3L may also be added to bone marrow cultures to produce large numbers of in vitro equivalents of the spleen DC subsets. Total DC, or their subset
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Isolation of Human Skin Dendritic Cell Subsets7–2596, 1993; Nestle et al., J Immunol 151:6535–6545, 1993). These factors led to the extensive use of skin DCs as the “prototype” migratory DCs in human studies. In this chapter, we detail the protocols to isolate DCs and resident macrophages from human skin. We also provide a multiparameter flow c
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