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Titlebook: Dendritic Cell Protocols; Shalin H. Naik Book 2010Latest edition Humana Press 2010 Activation.Antigen.Asthma.Immune System.Laboratory.Migr

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书目名称Dendritic Cell Protocols
编辑Shalin H. Naik
视频video
概述Provides step-by-step procedures for DC isolation from rodent and human tissue, as well as easy in vitro generation of equivalent cell types.Explores diverse assays to measure DC function in the conte
丛书名称Methods in Molecular Biology
图书封面Titlebook: Dendritic Cell Protocols;  Shalin H. Naik Book 2010Latest edition Humana Press 2010 Activation.Antigen.Asthma.Immune System.Laboratory.Migr
描述.Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, .Dendritic Cell Protocols, Second Edition. presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful .Methods in Molecular Biology.™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Comprehensive and cutting-edge, .De
出版日期Book 2010Latest edition
关键词Activation; Antigen; Asthma; Immune System; Laboratory; Migration; Transplantation; Vivo; gene expression; ge
版次2
doihttps://doi.org/10.1007/978-1-60761-421-0
isbn_softcover978-1-61779-676-0
isbn_ebook978-1-60761-421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2010
The information of publication is updating

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Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic ell development has improved (1, 2). Nonetheless, there are many gaps in our understanding, particularly regarding the early stages of development of hematopoietic progenitor cells (HPCs) into downstream lineage-biased and lineage-restricted precursors and the molecular mechanisms underlying these a
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Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantatione responses, including T-cell responses to transplanted organs. The inherent tolerogenicity of non-activated or “immature” DCs is well documented. Importantly, the infusion of DCs that are made resistant to activating inflammatory stimuli by “conditioning” through exposure to clinically approved imm
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Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccinesciple studies demonstrated immunogenicity and clinical responses were observed in a fraction of patients. Overlooking more than 200 publications one realizes, however, that it is almost impossible to compare many of these trials even in a given clinical setting or disease. This is primarily due to t
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CD8+, CD8−, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 LigandDCs for three decades. However, the most recent evidence suggests that these cultures represent the migratory and inflammatory DC subtypes and not the DC subtypes found in the steady state. By contrast a different culture method was described where mouse bone marrow is cultured with flt3 ligand for
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