书目名称 | Dendritic Cell Protocols | 编辑 | Shalin H. Naik | 视频video | | 概述 | Provides step-by-step procedures for DC isolation from rodent and human tissue, as well as easy in vitro generation of equivalent cell types.Explores diverse assays to measure DC function in the conte | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, .Dendritic Cell Protocols, Second Edition. presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful .Methods in Molecular Biology.™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Comprehensive and cutting-edge, .De | 出版日期 | Book 2010Latest edition | 关键词 | Activation; Antigen; Asthma; Immune System; Laboratory; Migration; Transplantation; Vivo; gene expression; ge | 版次 | 2 | doi | https://doi.org/10.1007/978-1-60761-421-0 | isbn_softcover | 978-1-61779-676-0 | isbn_ebook | 978-1-60761-421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2010 |
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