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Titlebook: Date Palm Biotechnology Protocols Volume I; Tissue Culture Appli Jameel M. Al-Khayri,S. Mohan‘Jain,Dennis V. Johnso Book 2017 Springer Scie

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Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Florauctive phase) to the vegetative state. The mature female inflorescence (fully developed) is cultured on MS induction medium containing 10 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 3 mg/L 2-isopentenyladenine (2iP), and 2 mg/L paclobutrazol (PBZ) or 2 mg/L abscisic acid (ABA). The basal part of th
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Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawateralms for cultivation. To meet this demand, tissue culture techniques have great potential for mass production of plantlets, especially using the indirect embryogenesis technique; any improvement of these techniques is a worthy objective. Low levels of salinity can enhance growth and development of t
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Indirect Somatic Embryogenesis from Mature Inflorescence Explants of Date Palmchapter focuses on the protocol for the induction of callus from inflorescence tissue, establishment for proliferation of somatic embryos, germination, elongation, rooting, and acclimatization. Female inflorescences, 30–40 cm in length, cv. Shaishee, were used for culture initiation. After disinfect
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Desiccation-Enhanced Maturation and Germination of Date Palm Somatic Embryos Derived from Cell Suspech is suitable to preserve existing natural genetic variability and propagation of variability generated from genetic improvement programs, including crossing, somaclonal variation, mutagenesis, and somatic hybridization. This chapter outlines a simplified protocol for date palm regeneration via som
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Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germinationembryos (SE) remains an impediment. This chapter focuses on two important physical factors to improve germination of date palm somatic embryos: the use of partial desiccation (3 h) of somatic embryos and the exposure to low temperature (4 °C for 24 h). High germination percentage (41%) is achieved b
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Histological Analysis of the Developmental Stages of Direct Somatic Embryogenesis Induced from In Vibing the ontogenesis of plant regeneration are limited. This chapter provides a simple protocol for the histological analysis of the successive developmental stages of direct somatic embryogenesis induced from in vitro leaf explants. Direct somatic embryos are obtained from Murashige and Skoog (MS)
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