Titlebook: DNAzymes; Methods and Protocol Gerhard Steger,Hannah Rosenbach,Ingrid Span Book 2022 The Editor(s) (if applicable) and The Author(s), under

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260232.jpg

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https://doi.org/10.1007/978-1-0716-2047-2biosensors; SELEX; DNA Aptamers; deoxyribozyme; FRET

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978-1-0716-2049-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Design of a DNAzymethat should help in the design of efficient DNAzymes and show some experimental methods to test the predictions. The main example is for a 10–23 DNAzyme, but the procedure works as well for the 8–17 DNAzyme family.

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Andrzej Małowiecki,Iwona Chomiak-Orsayribozymes, but is particularly important for performing in vitro selections. While the production of single-stranded RNA is straightforward by means of in vitro transcription, the enzymatic production of single-stranded DNA (ssDNA) on a preparative scale is often difficult. Nevertheless, several me

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