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Titlebook: DNA‘Protein Interactions; Principles and Proto Tom Moss Book 20012nd edition Humana Press 2001 DNA.RNA.Termination.Vivo.Western Blotting.ba

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书目名称DNA‘Protein Interactions
副标题Principles and Proto
编辑Tom Moss
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA‘Protein Interactions; Principles and Proto Tom Moss Book 20012nd edition Humana Press 2001 DNA.RNA.Termination.Vivo.Western Blotting.ba
描述Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
出版日期Book 20012nd edition
关键词DNA; RNA; Termination; Vivo; Western Blotting; bacteria; cell; development; gene; genes; nucleic acid; protein;
版次2
doihttps://doi.org/10.1385/1592592082
isbn_ebook978-1-59259-208-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2001
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https://doi.org/10.1007/978-3-031-52407-3living cells, it is often possible to establish the identity of these factors simply by comparison with the consensus binding sites of known factors such as Sp1, AP-1, NF-1, and so forth. The identity of each factor can then be confirmed using in vitro gel shift (electrophoretic mobility shift assay [EMSA]) or footprinting assays.
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In Vivo DNA Analysis,living cells, it is often possible to establish the identity of these factors simply by comparison with the consensus binding sites of known factors such as Sp1, AP-1, NF-1, and so forth. The identity of each factor can then be confirmed using in vitro gel shift (electrophoretic mobility shift assay [EMSA]) or footprinting assays.
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Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting,troblotting and analysis for sequence-specific DNA binding directly on the blot combines the advantages of a high-resolution fractionation step with the ability to rapidly analyze for a large number of different DNA-binding specificities.
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