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Titlebook: DNA Viruses; Methods and Protocol Paul M. Lieberman Book 2005 Humana Press 2005 DNA.PCR.Termination.cell lines.electron microscopy.gene exp

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书目名称DNA Viruses
副标题Methods and Protocol
编辑Paul M. Lieberman
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Viruses; Methods and Protocol Paul M. Lieberman Book 2005 Humana Press 2005 DNA.PCR.Termination.cell lines.electron microscopy.gene exp
描述A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.
出版日期Book 2005
关键词DNA; PCR; Termination; cell lines; electron microscopy; gene expression; genes; transcription; infectious di
版次1
doihttps://doi.org/10.1385/159259848X
isbn_softcover978-1-62703-810-2
isbn_ebook978-1-59259-848-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2005
The information of publication is updating

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AI U.S. Policies and Regulationstion. The reporter gene employed was green fluorescent protein (GFP) or secreted alkaline phosphatase (SEAP), whose assays offer real-time detection or quantification, respectively. This cell-based assay is simple, rapid, sensitive, specific, and quantitative and serves as a phenotypic method for determination of antiviral susceptibilities.
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https://doi.org/10.1007/978-3-031-29126-5e virions and are therefore valuable tools for the study of papillomavirus-cell interactions. The methods described can be adopted for other nonenveloped DNA viruses and may be useful for gene transfer.
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