Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D

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Chinese Entrepreneurs in Societyrification of fragments and preparation of strand-specific sequencing libraries. Deep sequencing of Okazaki fragments generates a comprehensive, genomic map of DNA synthesis, starting from a single asynchronous culture. Altogether this approach represents a tractable system to investigate key aspects of DNA replication and chromatin assembly.

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Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast,ine monophosphate, which can be used by the cell. This chapter describes the labeling of fission yeast, ., with the thymidine analog BrdU in order to identify sites and determine kinetics of DNA replication.

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Book 2015Latest editionfalls..Authoritative and cutting-edge, .DNA Replication: Methods and Protocols, Second Edition .provides a collections of methods intended for newcomers to this research field and for established laboratories..

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https://doi.org/10.1057/9781137492913 This allows quantitative measurements of several salient features of chromosome replication dynamics, such as fork velocity, fork asymmetry, inter-origin distances, and global instant fork density. In this chapter we describe how to obtain this information from asynchronous cultures of mammalian cells.

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