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Titlebook: DNA Repair Protocols; Lotte Bjergbæk Book 2012Latest edition Springer Science+Business Media New York 2012 Arabidopsis cell extracts.DNA r

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书目名称DNA Repair Protocols
编辑Lotte Bjergbæk
视频video
概述Covers both mammalian and non-mammalian model organisms.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplemen
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Repair Protocols;  Lotte Bjergbæk Book 2012Latest edition Springer Science+Business Media New York 2012 Arabidopsis cell extracts.DNA r
描述.Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third edition of .DNA Repair Protocols .covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful .Methods in Molecular Biology.? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. . .Thorough and intuitive, .DNA Repair Protocols, Third Edition .provides expert guidance for DNA repair, recombination, and replication..
出版日期Book 2012Latest edition
关键词Arabidopsis cell extracts; DNA repair; RNA; Saccharomyces cerevisiae; chromosomal DNA; electrophoretic mo
版次3
doihttps://doi.org/10.1007/978-1-61779-998-3
isbn_softcover978-1-4939-5954-9
isbn_ebook978-1-61779-998-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2012
The information of publication is updating

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Establishment of the DNA Repair-Defective Mutants in DT40 Cellsding efficient gene targeting and ease of chromosome manipulation. Although the genetic approach using the RNA interference technique has become the standard method particularly in human cells, DT40 still remains a powerful tool to investigate the regulation and function of genes and proteins in a v
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The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repairs technique, a small number of cells suspended in a thin agarose gel on a microscope slide is lysed, electrophoresed, and stained with a fluorescent DNA-binding dye. Cells with increased DNA damage display increased migration of chromosomal DNA from the nucleus towards the anode, which resembles the
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Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assayive effects of chemicals. The main advantage over the comet assay using cells of higher organisms is the genetic tractability and ease of cultivation of yeast. A drawback is the lower DNA content of the cells as well as the need for cell wall digestion prior to electrophoresis. Here, we describe in
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Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitativ nonmammalian species such as . (nematodes), . (fruit flies), and two species of fish (. and .). Since its development in the early 1990s (Kalinowski et al., Nucleic Acids Res 20:3485–3494, 1992; Salazar and Van Houten, Mutat Res 385:139–149, 1997; Yakes and Van Houten, Proc Natl Acad Sci USA 94:514
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In Vitro DNA Mismatch Repair in Human Cellstenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and t
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