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Titlebook: DNA Microarrays for Biomedical Research; Methods and Protocol Martin‘Dufva Book 20091st edition Humana Press 2009 Allele.DNA.Gene expressio

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书目名称DNA Microarrays for Biomedical Research
副标题Methods and Protocol
编辑Martin‘Dufva
视频video
概述Covers the use of DNA array from the largest commercial providers.Includes practical details to design and produce your own probes and arrays.Provides methods for gene expression profiling, SNP mutati
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Microarrays for Biomedical Research; Methods and Protocol Martin‘Dufva Book 20091st edition Humana Press 2009 Allele.DNA.Gene expressio
描述.DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In .DNA Microarrays for Biomedical Research: Methods and Protocols., experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful .Methods in Molecular Biology. series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls...Authoritative and highly practical,. DNA Microarrays for Biomedical Research: Methods and Protocols. presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex worl
出版日期Book 20091st edition
关键词Allele; DNA; Gene expression analysis; Genomic hybridization; Micoarray fabrication; Microarray; Mutation
版次1
doihttps://doi.org/10.1007/978-1-59745-538-1
isbn_softcover978-1-61737-941-3
isbn_ebook978-1-59745-538-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Comparative Genomic Hybridization: DNA Preparation for Microarray Fabrication,uct microarrays comprising large-insert clones available through genome sequencing projects. These methods are applicable to Bacterial and Phage Artificial Chromosomes (BAC and PAC) as well as fosmid and cosmid clones. The protocols are scalable for the construction of microarrays composed of several hundreds up to several ten thousands clones.
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Syllogizing in Red: Trivializing the Modalsication of DNA interactions and provide a powerful tool for genome-wide investigations. Immunoprecipitated DNA fragments associated with the desired protein are amplified, labelled and hybridized to DNA microarrays to detect enriched signals compared to a labelled reference sample.
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Comparative Genomic Hybridization: Microarray Design and Data Interpretation,r changes by array-CGH, as well as tools available for accurate analysis of array-CGH experiments. Although most methods listed here have been designed for microarrays comprising large-insert clones, they can be adapted easily to other types of microarray platforms, such as those constructed from printed or synthesized oligonucleotides.
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Chromatin Immunoprecipitation Using Microarrays,ication of DNA interactions and provide a powerful tool for genome-wide investigations. Immunoprecipitated DNA fragments associated with the desired protein are amplified, labelled and hybridized to DNA microarrays to detect enriched signals compared to a labelled reference sample.
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