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Titlebook: DNA Barcoding; Methods and Protocol Robert DeSalle Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sp

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书目名称DNA Barcoding
副标题Methods and Protocol
编辑Robert DeSalle
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Barcoding; Methods and Protocol Robert DeSalle Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sp
描述.This volume covers the latest advancements and methods used to study DNA barcoding. The chapters in this book are organized into six parts: theory, DNA manipulation, specialized barcoding methods, analysis of DNA barcodes, completing a successful DNA barcode project, and barcoding as citizen science. Part One looks at several theoretical issues in DNA barcoding concerned with DNA barcodes that can and should be used. Part Two discusses protocols for processing samples into DNA barcodes, including DNA isolation, sequencing and managing samples, and the resulting DNA barcodes. Part Three explores the next generation of DNA barcoding such as FRET-based detection, dual nanopore detection, skimming, and closed-tube methods. Part Four addresses programs and websites that handle DNA barcoding data, and includes character-based approaches, barcode gap methods, integrated approaches, and Barcode of Life Database. Part Five offers protocols for producing a successfulDNA barcoding project including plant and animal examples and protocols for generating a DNA Barcode Reference Library. Lastly, Part Six talks about several novel protocols for setting up an educational program using DNA barcode
出版日期Book 2024
关键词DNA Isolation; sequencing; Photo-vouchering; Min ION™‐based pipeline; NGSpeciesID
版次1
doihttps://doi.org/10.1007/978-1-0716-3581-0
isbn_softcover978-1-0716-3583-4
isbn_ebook978-1-0716-3581-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Simple, Robust Invertebrate DNA Barcoding: Chelex-Based DNA Extraction and Optimized , Amplificationthout specialized laboratory equipment, allowing them to be performed in classrooms or at home. Extracted DNA is stable in Chelex solution for at least a week at ambient temperature, allowing collection of DNA samples from remote students. These extractions provide quality DNA for many taxa and are
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Environmental DNA Isolation, Validation, and Preservation MethodsA, it is essential to avoid contamination from the point of collection through preservation and select a meaningful negative control. As eDNA can be obtained from a variety of samples and habitats (e.g., soil, water, air, or tissue), protocols will vary depending on usage. Samples may require additi
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