Titlebook: cDNA Library Protocols; Ian G. Cowell,Caroline A. Austin Book 1997 Springer Science+Business Media New York 1997

无畏

Book 1997wer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1—5 concentrate on cDNA library construction and manipula­ tion, Chapters 6 and 7 describe me

integral

phase-2-enzyme

1064-3745 se transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con­ structin

勉强

鞭打

Review of the Development of Energy Finance in a population of plants in response to environmental cues or at a defined stage of development. Therefore, large-scale extraction of RNA is the method of choice for preparations to be used for cDNA libraries.

大范围流行

Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Prihe different members of a gene family carry out related functions. A detailed protocol for the cloning by degenerate oligonucleotide polymerase chain reaction (PCR) of members of the . family of membrane water channels (., .) is discussed here.

Meditative

Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli,nged exposure of cells to CaCl. (.), substitution of Ca. ions by other cations, such as Rb. (.), Mn., and K., and addition of other compounds, such as dimethyl sulfoxide (DMSO), dithiothreitol, and cobalt hexaminechloride (.).

巧思

Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide double-stranded DNA probe containing the sequence recognized by the factor in question. Recombinants expressing a protein capable of binding the probe sequence in the presence of nonspecific competitor DNA are thus identified and can be isolated.

Emmenagogue

cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR,ary constructed from the entire tissue containing these cells, because in the latter case, genes expressed in the targeted cells may be too rare to be detected. cDNA clones of genes expressed in small amounts of material are often hard to obtain because the construction of conventional cDNA libraries requires microgram amounts of poly A. RNA (.).

船员

Isolation of Messenger RNA from Plant Tissues, in a population of plants in response to environmental cues or at a defined stage of development. Therefore, large-scale extraction of RNA is the method of choice for preparations to be used for cDNA libraries.