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Titlebook: Cytotoxic T-Cells; Methods and Protocol Elena Ranieri Book 2014 Springer Science+Business Media New York 2014 CTL exosomes.CTL studies.Cyto

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发表于 2025-3-21 18:05:44 | 显示全部楼层 |阅读模式
书目名称Cytotoxic T-Cells
副标题Methods and Protocol
编辑Elena Ranieri
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cytotoxic T-Cells; Methods and Protocol Elena Ranieri Book 2014 Springer Science+Business Media New York 2014 CTL exosomes.CTL studies.Cyto
描述​ In .Cytotoxic T-Cells: Methods and Protocols., leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Cytotoxic T-Cells: Methods and Protocols. seeks
出版日期Book 2014
关键词CTL exosomes; CTL studies; Cytotoxic T-Cells; Jack/stat pathway; Omics approaches; antigen T cell specifi
版次1
doihttps://doi.org/10.1007/978-1-4939-1158-5
isbn_softcover978-1-4939-4686-0
isbn_ebook978-1-4939-1158-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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Jay E. Johnson PhD,Dominique Broccoli PhDividuals but also to screen immunogenic antigens that could be considered as candidates for adoptive immunotherapeutic approaches. In vitro methods to expand CTL require culturing naïve T cells with antigen-presenting cells (APC) as stimulator cells that express specific antigens. Here, we describe
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App Development Using iOS iCloudpot allows the detection of low-frequency antigen-specific T cells that secrete cytokines and effector molecules, such as granzyme B and perforin. Cytotoxic T cell (CTL) studies have taken advantage with this high-throughput technology by providing insights into quantity and immune kinetics. Accurac
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App Development Using iOS iCloudiginate within the endocytic compartment by invagination of the endosome membrane. Therefore, they have a different biogenesis and molecular composition than microvesicles (>0.2 μm) shed from the plasma membrane. Although the functions of exosomes in vivo are beginning to be elucidated, increasing e
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Book Tracker Basic App Building effectively performed in CTL characterization to identify the location and distribution of target antigens in suspension cells or in tissues by staining with a specific antibody. The antibody may be conjugated to either a fluorescent or an enzymatic label, and the location of the label seen through
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App-Entwicklung für Mobile und Desktope over the last decade in areas such as cancer, immunology, neurological disorders, renal diseases, and many others, directed towards the new frontiers of personalized medicine and drug discovery..The following method covers a specific application of microarrays in the field of immunology, focusing
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