Titlebook: Cytoskeleton Methods and Protocols; Ray H. Gavin Book 20011st edition Humana Press 2001

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Microsequencing of Myosins for PCR Primer Design peptide sequences by standard protocols. For this reason, instead of identifying myosins first according to their biochemical activity and then obtaining their sequences, PCR and other DNA-based techniques exploiting the highly conserved sequences in the amino end, the “head” domain, have been used

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Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function of cytoskeletal filaments, both microtubules and actin filaments have been shown to support vesicle transport. The transport of vesicles is mediated by molecular motors and members of all three super-families of molecular motors-myosin, kinesin and cytoplasmic dynein-have been shown to function as

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Computer-Assisted Systems for the Analysis of Amoeboid Cell Motilityave evolved, flagellar or ciliary locomotion, and amoeboid locomotion. The latter represents the more general method of cellular locomotion. Amoeboid locomotion in the past has been trivialized. The view, strengthened by bad science fiction movies, was of a cytoplasmic mass, or blob, rolling blindly

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Evaluation of Individual-Cell Motilityon of a large number of molecular components including all main structures of the cytoskeleton (.,.). Thus, a detailed knowledge of the regulation and function of the cytoskeleton is of central importance for the understanding of cell motility, and conversely, investigations of cell motility may she

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