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Titlebook: Cytoskeleton; Methods and Protocol Ray H. Gavin Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive li

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Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cellsed to aggregate or disperse throughout the projections. Melanosome migration in RPE apical projections is dependent on actin filaments and thus renders this model system useful for investigations of actin-dependent organelle motility.
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https://doi.org/10.1007/978-3-540-69451-9 available (A, B, C, D, E, H, J), also making it a widely used compound for F-actin disruption. The same can be stated for latrunculins (A, B), derived from Red Sea sponges; however the mode of action is different by binding to G-actin and inhibiting incorporation into the filament. In the case of s
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Harald Fischer-Tiné,Christine Whyteudies on cilioprotists and other model organisms that raise specific questions regarding whether basal body- and centriole-associated nucleic acids, both DNA and RNA, should continue to be considered when seeking to employ cilioprotists as model systems for cytoskeletal research; and (3) new, mainly
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Imaging Cytoskeleton Components by Electron Microscopy) EM (PREM) for visualization of the cytoskeleton. The procedure is applicable to thin cultured cells growing on glass coverslips and consists of detergent extraction (or mechanical “unroofing”) of cells to expose their cytoskeleton, chemical fixation to provide stability, ethanol dehydration and cr
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Imaging the Cytoskeleton in Living Plant Rootsas ., the polymerized nutrient-supplemented agar is gently lifted or cut and used to secure pre-germinated seeds on the coverslip prior to root imaging. The agar-coverslip system we use for imaging the cytoskeleton in living roots along with general methods for expressing green fluorescent protein (
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