Titlebook: Cryopreservation in Assisted Reproduction; A Practitioner‘s Gui Zsolt Peter Nagy,Alex C. Varghese,Ashok Agarwal Book 2024 The Editor(s) (if

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Helmut Günzler,A. Müfit Bahadir,H. Wisser on slow freezing-rapid thawing with differential sucrose concentration (0.2 M in the freezing stage, 0.3 M in thawing stage) (Bianchi V et al., Reprod Biomed Online 14:64–71, 2007), describing the technique in detail, methods, management, organization, and safety of oocyte cryopreservation.

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Helmut Günzler,A. Müfit Bahadir,H. Wisser. However, recently, alternative sperm freezing methods have been developed including cryoprotectant-free vitrification and single sperm freezing. In this chapter, we aim to describe the various protocols for epididymal and testicular sperm processing and freezing that currently exist and compare the advantages and disadvantages of each method.

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Maximale Arbeitsplatzkonzentrationen (1987)es, ovarian tissue cryopreservation using slow freezing has proved effective in maintaining the viability of primordial follicles for further transplantation. For this reason, it is no longer considered an experimental procedure.

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Considerations in Immature Oocyte Cryopreservationminal vesicle (GV) stage, because the structure of cumulus-oocyte complex (COC) is damaged with the procedure of cryopreservation with immature oocytes, in which the structure of COC is essential during oocyte maturation, and potential for oocyte maturation is reduced when cryopreservation is performed at the immature stage.