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Titlebook: Cancer Cytogenetics and Cytogenomics; Methods and Protocol Jing Christine Ye,Henry H. Heng Book 2024 The Editor(s) (if applicable) and The

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书目名称Cancer Cytogenetics and Cytogenomics
副标题Methods and Protocol
编辑Jing Christine Ye,Henry H. Heng
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cancer Cytogenetics and Cytogenomics; Methods and Protocol Jing Christine Ye,Henry H. Heng Book 2024 The Editor(s) (if applicable) and The
描述.This detailed volume explores the frontiers of this new era in cancer cytogenetics and cytogenomics, focusing on establishing a karyotype as an information-based genomic framework, as well as presenting technological platforms for collecting and analyzing data at the genome level. It begins with several conceptual chapters that introduce ideas such as the Genome Architecture Theory, forcefully emphasizing the importance of cytogenomics in the post-genomics era. The book then proceeds with protocols covering both basic and advanced cytogenetic and cytogenomic methods, as well as diverse experiments beyond traditional cytogenetic platforms, and bioinformatics techniques and resources. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Authoritative and up-to-date, .Cancer Cytogenetics and Cytogenomics: Methods and Protocols. serves as an ideal guide to the unique power of this area of research in spatial biology and system-integrated geno
出版日期Book 2024
关键词Gene mutation; Karyotype dynamics; Genome Architecture Theory; Cancer evolution; Gene interaction networ
版次1
doihttps://doi.org/10.1007/978-1-0716-3946-7
isbn_softcover978-1-0716-3948-1
isbn_ebook978-1-0716-3946-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Methods of Detection and Mechanisms of Origin of Complex Structural Genome Variationsor “complex” (with more than two breakpoints). Studying the breakpoints of SVs at nucleotide resolution revealed additional, subtle structural variations, such that even “simple” SVs turned out to be “complex.” Genome-wide sequencing methods, such as fosmid and paired-end mapping, short-read and lon
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A Paradoxical Role for Somatic Chromosomal Mosaicism and Chromosome Instability in Cancer: Theoreticcompassing chromosomal mosaicism and instability) in cancer yields paradoxical results. Firstly, somatic mosaicism for specific chromosomal rearrangement causes cancer per se. Secondly, chromosomal mosaicism and instability are associated with a variety of diseases (chromosomal disorders demonstrati
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The Importance of Monitoring Non-clonal Chromosome Aberrations (NCCAs) in Cancer Researchromosome aberrations, or NCCAs, as insignificant noise. Our decade-long karyotype evolutionary studies have unexpectedly demonstrated otherwise. Not only the baseline of NCCAs is associated with fuzzy inheritance, but the frequencies of NCCAs can also be used to reliably measure genome or chromosome
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Cell Culture and Slide Preparation for Cytogenetic Studies of Hematological Neoplasmse essential steps to enrich and collect sufficient neoplastic cells for cytogenetic studies of the neoplasms. This chapter describes methods that are commonly used for culturing hematological neoplastic cells and preparing cytogenetic slides for clinical diagnosis and research of the neoplasms.
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Multitarget Fluorescence In Situ Hybridization Diagnostic Applications in Tumorsspectrally distinct fluorophore labels. The mFISH approach is currently a useful assay in the oncologic field for the detection of predictive, prognostic, and diagnostic biomarkers. In this chapter, we summarize the application of mFISH in the identification of target genetic aberrations in formalin
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High-Resolution FISH Analysis Using DNA Fibers Generated by Molecular Combingsepairs per micrometer. The combination of this approach with fluorescent in situ hybridization (FISH) has enabled the direct visualization of DNA structure and variations at an unprecedent high resolution. This technique has been successfully used in various studies such as the identification of co
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