Titlebook: Cancer Cell Dormancy; Methods and Protocol Marco Montagner Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

Hemoptysis

Ignacy Duleba,Iwona Karcz-Dulebain vivo effects on the local and systemic immune responses. While originally developed to characterize the effects of methotrexate and doxorubicin on murine 4T1 breast cancer cells and the relative immune response, the method can be broadened to other chemotherapies and syngeneic cancer models.

苦恼

In Vitro and In Vivo Systems to Study Tumor Dormancy and the Transition to Overt Metastases Inducedr dormant behavior (D2.0R, MCF7) or transient dormant metastatic behavior (D2A1) at a metastatic secondary site. Additionally, we present an in vitro and complementary in vivo system to study the switch from dormancy to metastatic growth driven by a fibrotic-like milieu enriched with the deposition of type I collagen.

冬眠

Bone Marrow Stroma Co-cultivation Model of Breast Cancer Dormancy,chniques. This manuscript describes the basic techniques for generating bone marrow stromal monolayers, co-incubating cancer cells and determining the effects on cancer cell proliferation and molecular signaling.

一小块

Establishing and Characterizing Chemotherapy-Induced Immunological Dormant Tumor Cell Lines,in vivo effects on the local and systemic immune responses. While originally developed to characterize the effects of methotrexate and doxorubicin on murine 4T1 breast cancer cells and the relative immune response, the method can be broadened to other chemotherapies and syngeneic cancer models.

挑剔小责

KISS1 and Kisspeptins Detection in Cell Lines, and social behaviors. These diverse functions point to KISS1 as a potential therapeutic molecule. Here we describe a methodology to detect KISS1 and KP from cell lysate and conditioned media in cell lines. This will serve as a critical tool to study KISS1 processing in KP.

痛恨

iodides

Antonio Celesti,Philipp Leitnerronment. Thus, studying the metabolic features of dormant disseminated tumor cells has gained importance in understanding the dormancy process. Here, we describe a method to perform metabolomics and .C tracer analysis in 3D cultures of dormant breast cancer cells.

reception

Advances in Service and Industrial Roboticsthe use of GFP/RFP:Luc2 reporters, we can monitor in vivo tumour growth and conduct ex vivo metastases assays to evaluate dormant metastatic cell harboring organs. Upon recovery of metastatic cells from ER. breast cancer models, downstream analyses can be conducted to assess the relationship between epithelial plasticity and metastatic dormancy.

留恋

https://doi.org/10.1007/978-3-030-00232-9g. In addition, a methodology for recovering brain metastatic cancer cells from the mouse brain is described, providing technical tips for unraveling the mysteries of cancer cell dormancy in brain metastasis.

松驰

Luiz Angelo Steffenel,Manuele Kirch Pinheirorom cisplatin chemotherapy. Many tools have been developed to monitor ROS production in cells in culture, while our ability to detect this in vivo remains limited. Here we describe a detailed protocol for determination of ROS in metastatic cells in the mouse lung tissue by detecting 4-hydroxy-2-noneal (4HNE) adducts formation in fixed tissues.