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Titlebook: Current Proteomic Approaches Applied to Brain Function; Enrique Santamaría,Joaquín Fernández-Irigoyen Book 2017 Springer Science+Business

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书目名称Current Proteomic Approaches Applied to Brain Function
编辑Enrique Santamaría,Joaquín Fernández-Irigoyen
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概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Neuromethods
图书封面Titlebook: Current Proteomic Approaches Applied to Brain Function;  Enrique Santamaría,Joaquín Fernández-Irigoyen Book 2017 Springer Science+Business
描述.This volume focuses on protein analysis covering a wide spectrum of the utility of mass spectrometry within neurobiological disciplines. The chapters in this book discuss label (iTRAQ, TMT, protein arrays) and label-free workflows (SWATH, MALDI Imaging, and label-free quantitation). Other chapters look at experimental strategies targeted to the identification and quantitation of specific lipids and post-translational modifications, as well as proteomic workflows that focus on characterization of subcellular proteomes. The last few chapters in the book describe various bioinformatics pipelines used to analyze the molecular data derived from high-throughput transcriptomic and proteomic experiments on brain tissue. The .Neuromethods .series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals..Thorough and comprehensive, .Current Proteomic Approaches Applied to Brain Function. is a valuable resource for graduate students and postdoctoral fellows interested in neuroproteomics, as well as researchers looking for further insight into the growing field of mass spectrometry in neuroscience.. . .
出版日期Book 2017
关键词mass spectrometry; neurodegenerative disease; fluorescence activated nuclei sorting; glycoproteomics; ne
版次1
doihttps://doi.org/10.1007/978-1-4939-7119-0
isbn_softcover978-1-4939-8390-2
isbn_ebook978-1-4939-7119-0Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media LLC 2017
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Protein Microarrays in Neurodegenerative Diseasesed by these set of diseases, leading to the final neuronal cell death, they are only partially understood. Therefore, there is an urgent need to find and elucidate molecular mechanisms underlying the formation and progression of these diseases to get an early diagnosis and find new therapeutic targe
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Nontargeted Brain Lipidomic Profiling Performed by UPLC-ESI-qToF-MS/MSon of lipid molecular species and of their biological roles with respect to expression of proteins involved in lipid metabolism and function, including gene regulation.” Lipids play diverse roles in brain cellular function which is reflected by an enormous variation in the structures of lipid molecu
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Neuroproteomics Using Short GeLC-SWATH: From the Evaluation of Proteome Changes to the Clarificationme modulation of the nervous system. Although there are several quantitative mass spectrometry approaches, all of them still require the digestion of the proteins into peptides making this step critical for the success of this type of analysis. By turning into quantitative, these methods are not onl
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Analysis of Brain Phosphoproteome Using Titanium Dioxide Enrichment and High-Resolution LC-MS/MSeptide enrichment, and peptide identification and quantification by LC-MS/MS. We describe a refined method for rapid, simple, and efficient TiO.-based phosphopeptide enrichment that relies on specific binding of the peptidyl phosphate group and TiO., with free phosphate competitor added to reduce no
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