Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harriëtte Oldenhof Book 2015Latest edition Springer Science+Business Media

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lactic

Timothy Kerswell,Surendra Pratap the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.

ELUDE

on a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175–185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.

即席

rom sperm samples, and for selecting morphologically normal motile sperm. The methods that are described here include: ordinary centrifugation, sperm swim-up, glass wool and Sephadex filtration/adherence, and single-layer as well as discontinuous two-layer iodixanol density gradient centrifugation.

万神殿

1064-3745 ation advice from the experts.Includes supplementary materia.This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-dr

ARBOR

ARBOR

Book 2015Latest editionrs focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written

空气

白杨鱼

合法

he different cooling rates needed in different temperature regimes during the freezing process are being described. Cryopreservation procedures are described in case of using both specialized automated equipment and simple equipment.