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Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich Förster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s),

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发表于 2025-3-21 17:34:16 | 显示全部楼层 |阅读模式
书目名称Cryo-Electron Tomography
副标题Structural Biology i
编辑Friedrich Förster,Ariane Briegel
视频videohttp://file.papertrans.cn/241/240489/240489.mp4
概述Comprehensive description of cryo-electron tomography.Addresses the underlying principles and all recent developments of cryo-ET.A valuable resource for scientists working in the field of structural b
丛书名称Focus on Structural Biology
图书封面Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich Förster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s),
描述.This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET.. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics,
出版日期Book 2024
关键词electron optics; phase contrast; tomography; tomographic reconstruction; Fourier-slice theorem; cryo-prep
版次1
doihttps://doi.org/10.1007/978-3-031-51171-4
isbn_softcover978-3-031-51173-8
isbn_ebook978-3-031-51171-4Series ISSN 1571-4853 Series E-ISSN 2542-9566
issn_series 1571-4853
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl
1 Front Matter
Abstract
2 ,Fundamentals of Instrumentation and Electron Optics for Cryo-Electron Tomography, Juergen M. Plitzko,Daniel Bollschweiler
Abstract
3 ,Principles of Tomographic Reconstruction, Achilleas S. Frangakis
Abstract
4 ,Cryogenic Preparations of Biological Specimens for Cryo-Electron Tomography, Edoardo D’Imprima,Herman K. H. Fung,Ievgeniia Zagoriy,Julia Mahamid
Abstract
5 ,Cryo-Focused Ion Beam Milling of Cells, Digvijay Singh,Elizabeth Villa
Abstract
6 ,In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques, Sven Klumpe,Philipp S. Erdmann
Abstract
7 ,Targeting, Localisation and Identification in Cryo-ET, Rainer Kaufmann,Kay Grünewald,Lindsay A. Baker
Abstract
8 ,Automated Cryo Electron Tomography Data Collection, Wim J. H. Hagen
Abstract
9 ,Subtomogram Averaging,
Abstract
10 ,High-Resolution Tomography, Molecular Model Building and Visualization, Alberto Bartesaghi
Abstract
11 ,Beam-Induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electro Shawn Zheng,Axel Brilot,Yifan Cheng,David A. Agard
Abstract
12 ,Detection and Interpretation of Cellular Structures in Tomograms: Segmentation, Localization and Sp Antonio Martinez-Sanchez,Vladan Lučić
Abstract
13 ,Electron Tomography of Cryo-Fixed and Resin-Embedded Samples, Alicia C. Borgeaud,Alejandro Melero,Lazar Ivanović,Wanda Kukulski
Abstract
14 ,After the Microscope: Long-Term Care of Electron Tomography Data, Catherine M. Oikonomou,Grant J. Jensen
Abstract
书目名称Cryo-Electron Tomography影响因子(影响力)




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发表于 2025-3-22 00:09:06 | 显示全部楼层
Petra Lietz,Molie Tobin,Dita Nugroho. We then provide a comprehensive account of the current state-of-the-art cryo-ET sample preparation techniques for specimens ranging from in-vitro reconstitutions, single cells, to 3D cell cultures, and whole multicellular organisms. We point out specific considerations that facilitate a wide range
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Shauna Butterwick,Marilou Carrilloachine learning and advanced algorithms, and discuss challenges posed by the recent developments of deep learning methods. New approaches for localization of molecular complexes and spatial analysis are expected to provide precise molecular maps of diverse cellular compartments, elucidate the organi
发表于 2025-3-23 00:15:17 | 显示全部楼层
Telling Old Stories in New Waysg a wider context with equal detail. Electron tomography can also be performed on serial sections which allows entire cells or tissue volumes to be reconstructed at high resolution. Further, high precision correlative microscopy workflows can be implemented, permitting to combine information on mole
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Fundamentals of Instrumentation and Electron Optics for Cryo-Electron Tomography, eventually recorded in the image plane—by ever more sophisticated detectors. As we acknowledge that our sample fries all too quickly, we l also discuss low dose techniques and phase plates. Finally, we provide a short outlook towards exciting developments that may well push cryo-electron tomography
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