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Titlebook: Coronaviruses; Methods and Protocol Helena Jane Maier,Erica Bickerton,Paul Britton Book 2015 Springer Science+Business Media New York 2015

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发表于 2025-3-21 19:53:33 | 显示全部楼层 |阅读模式
书目名称Coronaviruses
副标题Methods and Protocol
编辑Helena Jane Maier,Erica Bickerton,Paul Britton
视频videohttp://file.papertrans.cn/239/238357/238357.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Coronaviruses; Methods and Protocol Helena Jane Maier,Erica Bickerton,Paul Britton Book 2015 Springer Science+Business Media New York 2015
描述.This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize
出版日期Book 2015
关键词coronovirology; titration techniques; viral protein; virus genomes; virus pathogenicity; virus replicatio
版次1
doihttps://doi.org/10.1007/978-1-4939-2438-7
isbn_softcover978-1-4939-4921-2
isbn_ebook978-1-4939-2438-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

书目名称Coronaviruses影响因子(影响力)




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书目名称Coronaviruses网络公开度




书目名称Coronaviruses网络公开度学科排名




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书目名称Coronaviruses被引频次学科排名




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书目名称Coronaviruses年度引用学科排名




书目名称Coronaviruses读者反馈




书目名称Coronaviruses读者反馈学科排名




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发表于 2025-3-21 23:53:54 | 显示全部楼层
Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomicsent responsible for the fulminating disease of guinea fowl, we identified a novel avian gammacoronavirus associated with the disease using metagenomics. Next-generation sequencing is an unbiased approach that allows the sequencing of virtually all the genetic material present in a given sample.
IVORY
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Awning
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Ganglion
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发表于 2025-3-22 10:21:55 | 显示全部楼层
The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
FORGO
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发表于 2025-3-22 13:12:35 | 显示全部楼层
Conquest
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Isolation and Propagation of Coronaviruses in Embryonated Eggso and its membranes provide the diversity of cell types that are needed for successful replication of a wide variety of different viruses. Within the family . the embryonated egg has been used as a host system primarily for two avian coronaviruses within the genus ., infectious bronchitis virus (IBV
Conquest
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Organization
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ADAGE
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Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitihe IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert an
Nomogram
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