Titlebook: Corneal Regeneration; Methods and Protocol Mark Ahearne Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 limbal

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Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant, limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it is possible to obtain up to three limbal epithelial primary cultures from the same explant, thus increasing the efficiency of the in vitro cell culture.

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A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture,scribe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for multiple downstream applications which are currently unattainable using traditional in vitro techniques.

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Some More Advanced STEM Problems,face area, accessibility, immune privilege, avascularity, and ease of visualization. Herein, we describe the design, testing, and delivery of Cas9 and guide RNAs to target genes expressed in the cornea.

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Some More Advanced STEM Problems,iological processes, resulting in enhanced and accelerated extracellular matrix deposition. Herein, we describe the ex vivo formation of corneal stromal-like assemblies using human corneal fibroblasts and macromolecular crowding.

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https://doi.org/10.1007/978-1-0716-0599-8limbal stem cell; Immunomodulation; collagen scaffolds; corneal repair; endothelial keratoplasty