Titlebook: Conditional Mutagenesis: An Approach to Disease Models; Robert Feil,Daniel Metzger Book 2007 Springer-Verlag Berlin Heidelberg 2007 Chromo

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Tetracycline-Controlled Genetic Switchesse. Trancriptional activation can be induced by activators termed tTA (Tet-Off) or rtTA (Tet-On) in the absence and presence of Tet, respectively. The Tet-Off and Tet-On systems are complementary, and the decision to choose one over the other depends on the particular experimental strategy. Both sys

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Novel Gene Switchesery as well as fundamental and applied research. This chapter provides a comprehensive overview of the different approaches for regulating gene activity and product protein formation at different biosynthetic levels, from genomic rearrangements over transcription and translation control to strategie

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Improved Embryonic Stem Cell Technologiesadvances, including tetraploid aggregation, new site-specific recombinases and RNAi, have enabled more sophisticated manipulation of the ES cell genome. For instance, it is now possible to control gene expression in both a temporally and spatially restricted manner. Such new technologies are answeri

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Gene Trap Mutagenesiso a powerful tool for dissecting the genetic causes of human disease and identifying potential targets for pharmaceutical intervention. With the recent sequencing of the human and mouse genomes, a large number of novel genes have been identified whose function in normal and disease physiology remain

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Titlebook: Conditional Mutagenesis: An Approach to Disease Models; Robert Feil,Daniel Metzger Book 2007 Springer-Verlag Berlin Heidelberg 2007 Chromo

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